Journal: Frontiers in Immunology

Article Title: Antigen Presenting Cells Contribute to Persistent Immune Activation Despite Antiretroviral Therapy Initiation During Hyperacute HIV-1 Infection

doi: 10.3389/fimmu.2021.738743

Figure Lengend Snippet: pDC cytokine production in response to TLR antigen stimulation. HIV negative individuals and hyperacute and chronic ART groups at month 1, 12 and 24 post-infection were assessed for pDC TNF-α production following stimulation of (A) TLR4, (B) TLR7/8 or (C) TLR9 and IFN-α production following stimulation of (D) TLR4, (E) TLR7/8 or (F) TLR9.

Article Snippet: Toll-like receptor (TLR) agonists for cell stimulation were as follows: TLR4 ligand lipopolysaccharide (LPS) was from Sigma-Aldrich (Saint Louis, MO, USA), TLR7/8 ligand imidazoquinoline compound (CL097) and TLR9 ligand class A CpG oligonucleotide (ODN 2216) were from InvivoGen (San Diego, CA, USA).

Techniques: Infection

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: 3-Day monocyte-derived dendritic cells stimulated with a combination of OK432, TLR7/8 ligand, and prostaglandin E 2 are a promising alternative for cancer immunotherapy

doi: 10.1007/s00262-018-2216-y

Figure Lengend Snippet: DC matured with PGE2 migrate towards CCL19. 50,000 DC were placed in the upper chamber of a transwell plate and incubated for 4 h. Approximately 20% of DC matured with the cytokine cocktail (ccDC) migrated towards CCL19, while approximately 10% of OK432 + CL097 + PGE2 matured DC could be collected in the lower chamber. DC matured without PGE2 did not show considerable chemotaxis towards CCL19. IDC: immature DC; ccDC: DC matured with Jonuleit cytokine cocktail TNFα, IL-6, IL-1β, and PGE2; OK432: DC matured with 0.1 KE/ml OK432; OK432 + CL097: DC matured with 0.1 KE/ml OK432 and CL097; OK432 + CL097 + PGE2: DC matured with 0.1 KE/ml OK432, CL097 and 0.5 µg/ml PGE2. Each symbol represents a different donor

Article Snippet: Cytokines were replenished 24 h before harvesting, and DC were matured using OK432 (Picibanil; 0.1 KE/ml, Chugai Pharmaceutical Co. Ltd, Tokyo, Japan), TLR7/8 ligand (CL097; 1 μg/ml; Invivogen, catalogue number tlrl-c97) and PGE 2 , 0.5 μg/ml (Sigma-Aldrich, catalogue number P0409).

Techniques: Incubation, Chemotaxis Assay

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: 3-Day monocyte-derived dendritic cells stimulated with a combination of OK432, TLR7/8 ligand, and prostaglandin E 2 are a promising alternative for cancer immunotherapy

doi: 10.1007/s00262-018-2216-y

Figure Lengend Snippet: DC matured with a cocktail consisting of OK432, CL097, and PGE2 express high amounts of co-stimulatory molecules and CCR7. HLA-DR was highly expressed on all cell populations. CD83 was increased in all matured DC populations. CCR7 was significantly increased (p < 0.05) in every group matured with PGE2 compared to iDC. CD80 was significantly higher expressed in ccDC and DC treated with OK432, CL097, and PGE2 (p < 0.05) compared to iDC, while the increase of CD86 expression did not reach statistical significance. IDC immature DC, ccDC: DC matured with Jonuleit cytokine cocktail TNF, IL-6, IL-1β, and PGE2; OK432: DC matured with 0.1 KE/ml OK432; OK432 + CL097: DC matured with 0.1 KE/ml OK432 and CL097; OK432 + CL097 + PGE2: DC matured with 0.1 KE/ml OK432, CL097 and 0.5 µg/ml PGE2; MFI: median fluorescence intensity. Each symbol represents a different donor

Article Snippet: Cytokines were replenished 24 h before harvesting, and DC were matured using OK432 (Picibanil; 0.1 KE/ml, Chugai Pharmaceutical Co. Ltd, Tokyo, Japan), TLR7/8 ligand (CL097; 1 μg/ml; Invivogen, catalogue number tlrl-c97) and PGE 2 , 0.5 μg/ml (Sigma-Aldrich, catalogue number P0409).

Techniques: Expressing, Fluorescence

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: 3-Day monocyte-derived dendritic cells stimulated with a combination of OK432, TLR7/8 ligand, and prostaglandin E 2 are a promising alternative for cancer immunotherapy

doi: 10.1007/s00262-018-2216-y

Figure Lengend Snippet: OK432-stimulated DC secrete more cytokines relevant for Th1 cell stimulation. All OK432-matured DC had a significant increase in IL-12p70 secretion compared to ccDC and iDC that was further elevated with the addition of CL097, but decreased by PGE2. The amount of IL-12p70 was reduced by the addition of PGE2, but the cells still produced significantly more IL-12p70 than ccDC (p < 0.05). This pattern was also observed with RANTES/CCL5, MIP-1α/CCL3 and MIP-1β /CCL4. IL-12p40 was highly elevated by the combination of OK432, CL097 and PGE2, albeit not statistically significant compared to other matured DC groups. IDC: immature DC; ccDC: DC matured with Jonuleit cytokine cocktail TNFα, IL-6, IL-1β, and PGE2; OK432: DC matured with 0.1 KE/ml OK432; OK432 + CL097: DC matured with 0.1 KE/ml OK432 and CL097; OK432 + CL097 + PGE2: DC matured with 0.1 KE/ml OK432, CL097, and 0.5 µg/ml PGE2. Each symbol represents a different donor

Article Snippet: Cytokines were replenished 24 h before harvesting, and DC were matured using OK432 (Picibanil; 0.1 KE/ml, Chugai Pharmaceutical Co. Ltd, Tokyo, Japan), TLR7/8 ligand (CL097; 1 μg/ml; Invivogen, catalogue number tlrl-c97) and PGE 2 , 0.5 μg/ml (Sigma-Aldrich, catalogue number P0409).

Techniques: Cell Stimulation, Produced

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: 3-Day monocyte-derived dendritic cells stimulated with a combination of OK432, TLR7/8 ligand, and prostaglandin E 2 are a promising alternative for cancer immunotherapy

doi: 10.1007/s00262-018-2216-y

Figure Lengend Snippet: Increased IL-12p70 secretion by DC upon addition of TLR ligand containing maturation cocktails. 3-day DC were matured with different maturation cocktails containing TLR ligands: (i) Kalinski cocktail [35]: TNF, IL-1β, IFN-α, IFN-γ, and polyI:C; (ii) Lövgren cocktail [36]: TNF, IFN-γ, R848, and polyI:C; (iii) Zobywalski cocktail [37]: TNF, IL-1β, IFN-γ, PGE2, R848, and polyI:C. IDC: immature DC; ccDC: DC matured with Jonuleit cytokine cocktail TNFα, IL-6, IL-1β, and PGE2; OK432: DC matured with 0.1 KE/ml OK432; OK432 + CL097: DC matured with 0.1 KE/ml OK432 and CL097; OK432 + CL097 + PGE2: DC matured with 0.1 KE/ml OK432, CL097, and 0.5 µg/ml PGE2. Each symbol represents a different donor

Article Snippet: Cytokines were replenished 24 h before harvesting, and DC were matured using OK432 (Picibanil; 0.1 KE/ml, Chugai Pharmaceutical Co. Ltd, Tokyo, Japan), TLR7/8 ligand (CL097; 1 μg/ml; Invivogen, catalogue number tlrl-c97) and PGE 2 , 0.5 μg/ml (Sigma-Aldrich, catalogue number P0409).

Techniques:

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: 3-Day monocyte-derived dendritic cells stimulated with a combination of OK432, TLR7/8 ligand, and prostaglandin E 2 are a promising alternative for cancer immunotherapy

doi: 10.1007/s00262-018-2216-y

Figure Lengend Snippet: OK432-matured DC are superior at inducing T-cell proliferation. 2 × 105 CFDA-SE-stained autologous NAC (PBMC depleted of monocytes) were co-cultured with 5 × 104 DC matured with noted stimuli for 5 days. Cells were measured for fluorescence intensity to determine proliferation. OK432 stimulated DC induced more T-cell proliferation than ccDC. IDC: immature DC; ccDC: DC matured with Jonuleit cytokine cocktail TNFα, IL-6, IL-1β, and PGE2; OK432: DC matured with 0.1 KE/ml OK432; OK432 + CL097: DC matured with 0.1 KE/ml OK432 and CL097; OK432 + CL097 + PGE2: DC matured with 0.1 KE/ml OK432, CL097 and 0.5 µg/ml PGE2. Each symbol represents a different donor

Article Snippet: Cytokines were replenished 24 h before harvesting, and DC were matured using OK432 (Picibanil; 0.1 KE/ml, Chugai Pharmaceutical Co. Ltd, Tokyo, Japan), TLR7/8 ligand (CL097; 1 μg/ml; Invivogen, catalogue number tlrl-c97) and PGE 2 , 0.5 μg/ml (Sigma-Aldrich, catalogue number P0409).

Techniques: Staining, Cell Culture, Fluorescence

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: 3-Day monocyte-derived dendritic cells stimulated with a combination of OK432, TLR7/8 ligand, and prostaglandin E 2 are a promising alternative for cancer immunotherapy

doi: 10.1007/s00262-018-2216-y

Figure Lengend Snippet: OK432-matured DC are able to induce antigen-specific T cells. Autologous NAC (PBMC depleted of monocytes) were co-cultured with PPD-loaded DC matured with noted stimuli for 7 days, and antigen-specific IFN-gamma secretion by CD4 + and CD8 + cells was analyzed by flow cytometry with ccDC as stimulators (+/− PPD). In some experiments (donors 12–14), the induced NAC were rested for 5 days prior to the IFN-g secretion assay. ccDC: DC matured with Jonuleit cytokine cocktail TNFα, IL-6, IL-1β, and PGE2; OK432 + CL097 + PGE2: DC matured with 0.1 KE/ml OK432, CL097, and 0.5 µg/ml PGE2. Each symbol represents a different donor

Article Snippet: Cytokines were replenished 24 h before harvesting, and DC were matured using OK432 (Picibanil; 0.1 KE/ml, Chugai Pharmaceutical Co. Ltd, Tokyo, Japan), TLR7/8 ligand (CL097; 1 μg/ml; Invivogen, catalogue number tlrl-c97) and PGE 2 , 0.5 μg/ml (Sigma-Aldrich, catalogue number P0409).

Techniques: Cell Culture, Flow Cytometry

Journal: Chinese Journal of Cancer Research

Article Title: Impact of 5-Fu/oxaliplatin on mouse dendritic cells and synergetic effect with a colon cancer vaccine

doi: 10.21147/j.issn.1000-9604.2018.02.03

Figure Lengend Snippet: Effect of 5-Fu/oxaliplatin (OX) on dendritic cell (DC) phenotypes. The murine myeloid-derived DCs were cultured in vitro, and Toll-like receptor 3 (TLR3), TLR7/8, 500 μg/mL 5-Fu and/or 10 μg/mL oxaliplatin were added at the last 48 h. Flow cytometry was used to detect the phenotypic expression of DCs in each group. imDCs: immature DCs, no TLR ligand and/or chemotherapeutic drugs were added; mDC: mature DCs, combined TLR ligands were added. Data are obtained from three independent experiments and represented as . *, P<0.05; **, P<0.01; ns, no significance.

Article Snippet: On d 6, 20 μg/mL of TLR3 ligand Poly (I:C) and 3 μg/mL of TLR7/8 ligand CL097 (Invitrogen) ( 10 ) in combination with 500 μg/mL of 5-Fu and/or 10 μg/mL of oxaliplatin (OX) (Sigma-Aldrich, St Louis, MO, USA) were added into the culture.

Techniques: Derivative Assay, Cell Culture, In Vitro, Flow Cytometry, Expressing

Journal: Chinese Journal of Cancer Research

Article Title: Impact of 5-Fu/oxaliplatin on mouse dendritic cells and synergetic effect with a colon cancer vaccine

doi: 10.21147/j.issn.1000-9604.2018.02.03

Figure Lengend Snippet: Effect of 5-Fu/oxaliplatin on the expression of dendritic cell (DC) programmed death ligand 1/2 (PD-L1/L2). The murine myeloid-derived DCs were cultured in vitro, and the expression of the inhibitory receptor PD-L1/L2 on the DC surface was detected. (A) The expression of PD-L1 in each group; (B) The expression of PD-L2 in each group. The flow chart indicates a typical experimental sample derived from three independent experiments. Data are obtained from three independent experiments and represented as . *, P<0.05; **, P<0.01; ns, no significance.

Article Snippet: On d 6, 20 μg/mL of TLR3 ligand Poly (I:C) and 3 μg/mL of TLR7/8 ligand CL097 (Invitrogen) ( 10 ) in combination with 500 μg/mL of 5-Fu and/or 10 μg/mL of oxaliplatin (OX) (Sigma-Aldrich, St Louis, MO, USA) were added into the culture.

Techniques: Expressing, Derivative Assay, Cell Culture, In Vitro

Journal: Chinese Journal of Cancer Research

Article Title: Impact of 5-Fu/oxaliplatin on mouse dendritic cells and synergetic effect with a colon cancer vaccine

doi: 10.21147/j.issn.1000-9604.2018.02.03

Figure Lengend Snippet: Effects of 5-Fu/oxaliplatin on the secretion of dendritic cell (DC) cytokines. A small amount of supernatant was obtained during DC harvesting, and the cytokines were detected by cytometric bead array (CBA). The data are obtained from three independent experiments and represented as . *, P<0.05; **, P<0.01; ***, P<0.001; ns, no significance.

Article Snippet: On d 6, 20 μg/mL of TLR3 ligand Poly (I:C) and 3 μg/mL of TLR7/8 ligand CL097 (Invitrogen) ( 10 ) in combination with 500 μg/mL of 5-Fu and/or 10 μg/mL of oxaliplatin (OX) (Sigma-Aldrich, St Louis, MO, USA) were added into the culture.

Techniques:

Journal: Chinese Journal of Cancer Research

Article Title: Impact of 5-Fu/oxaliplatin on mouse dendritic cells and synergetic effect with a colon cancer vaccine

doi: 10.21147/j.issn.1000-9604.2018.02.03

Figure Lengend Snippet: Effects of 5-Fu/oxaliplatin on the phenotypes of dendritic cells (DCs) in tumor bearing mice. MC38 colon cancer cells were subcutaneously inoculated in mice, and when the tumor volume reached 500 mm3, 50 mg/kg 5-Fu and/or 5 mg/kg of oxaliplatin were intraperitoneally injected. Following 5 d of treatment, the DCs of the spleens, tumor tissues and draining lymph nodes were separated, and the phenotype expression was detected by flow cytometry. (A) The phenotypic expression of DCs in the spleens; (B) The phenotypic expression of DCs in the tumor tissues; (C) The phenotypic expression of DCs in the lymph nodes. The data are obtained from two independent experiments and represented the .

Article Snippet: On d 6, 20 μg/mL of TLR3 ligand Poly (I:C) and 3 μg/mL of TLR7/8 ligand CL097 (Invitrogen) ( 10 ) in combination with 500 μg/mL of 5-Fu and/or 10 μg/mL of oxaliplatin (OX) (Sigma-Aldrich, St Louis, MO, USA) were added into the culture.

Techniques: Injection, Expressing, Flow Cytometry

Journal: Chinese Journal of Cancer Research

Article Title: Impact of 5-Fu/oxaliplatin on mouse dendritic cells and synergetic effect with a colon cancer vaccine

doi: 10.21147/j.issn.1000-9604.2018.02.03

Figure Lengend Snippet: The specific activation of lymphocytes by 5-Fu/oxaliplatin in vivo. When the volume of the subcutaneous tumor reached 500 mm3, 50 mg/kg 5-Fu and/or 5 mg/kg oxaliplatin were injected in mice. The spleens, tumor tissues and draining lymph nodes were removed 5 d following the injection. The tumor infiltrating leukocytes, splenocytes and lymph node cells were co-cultured with nonviable MC38 cells for 16 h. Enzyme-linked immunosorbent assay (ELISA) was used to detect interferon-γ (IFN-γ) secretion. (A) Secretion level of IFN-γ from tumor infiltrating leukocytes; (B) Secretion level of IFN-γ from splenocytes; (C) Secretion level of IFN-γ from lymph node cells. The data are obtained from three independent experiments and presented as . *, P<0.05; **, P<0.01; ***, P<0.001; ns, no significance.

Article Snippet: On d 6, 20 μg/mL of TLR3 ligand Poly (I:C) and 3 μg/mL of TLR7/8 ligand CL097 (Invitrogen) ( 10 ) in combination with 500 μg/mL of 5-Fu and/or 10 μg/mL of oxaliplatin (OX) (Sigma-Aldrich, St Louis, MO, USA) were added into the culture.

Techniques: Activation Assay, In Vivo, Injection, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Chinese Journal of Cancer Research

Article Title: Impact of 5-Fu/oxaliplatin on mouse dendritic cells and synergetic effect with a colon cancer vaccine

doi: 10.21147/j.issn.1000-9604.2018.02.03

Figure Lengend Snippet: The growth curve of MC38 xenografts in mice following chemotherapy combined with immunotherapy. When the volume of the tumor reached 500 mm3, the chemotherapeutic drugs 5-Fu/oxaliplatin and/or phosphate buffered saline (PBS) were intraperitoneally injected in mice. The immune therapies were conducted twice on d 3 and d 7 following injection of the chemotherapeutic drugs [intravenous injection of Toll-like receptor 9 (TLR9) ligand and CD1d-MC38/α-galactosylceramide (α-GC) compound, respectively]. A total of 6 mice were used in each group. The inoculated tumors were observed every 2 or 3 d. The maximum and minimum diameters of the tumors were measured in order to calculate the tumor volume. (A) Chemotherapy for MC38 xenografts in mice; (B) Chemotherapy combined with immunotherapy for MC38 xenografts in mice. *, P<0.05; **, P<0.01; ***, P<0.001; ns, no significance.

Article Snippet: On d 6, 20 μg/mL of TLR3 ligand Poly (I:C) and 3 μg/mL of TLR7/8 ligand CL097 (Invitrogen) ( 10 ) in combination with 500 μg/mL of 5-Fu and/or 10 μg/mL of oxaliplatin (OX) (Sigma-Aldrich, St Louis, MO, USA) were added into the culture.

Techniques: Injection

Journal: Chinese Journal of Cancer Research

Article Title: Impact of 5-Fu/oxaliplatin on mouse dendritic cells and synergetic effect with a colon cancer vaccine

doi: 10.21147/j.issn.1000-9604.2018.02.03

Figure Lengend Snippet: The survival curves of mice bearing MC38 xenografts following chemotherapy combined with immunotherapy treatment. When the volume of the tumor reached 500 mm3, the chemotherapeutic drugs 5-Fu/oxaliplatin and/or phosphate buffered saline (PBS) were intraperitoneally injected in the mice. The immune therapies were conducted twice on d 3 and d 7 following injection of the chemotherapeutic drugs. The survival curves of the tumor bearing mice were drawn by the Kaplan-Meier method. The tumor bearing mice were observed for 90 d after inoculation. The mice with a tumor volume of greater than 5,000 mm3 were sacrificed.

Article Snippet: On d 6, 20 μg/mL of TLR3 ligand Poly (I:C) and 3 μg/mL of TLR7/8 ligand CL097 (Invitrogen) ( 10 ) in combination with 500 μg/mL of 5-Fu and/or 10 μg/mL of oxaliplatin (OX) (Sigma-Aldrich, St Louis, MO, USA) were added into the culture.

Techniques: Injection